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pgl3-basic firefly luciferase expression vector  (Promega)

 
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    Promega pgl3-basic firefly luciferase expression vector
    Pgl3 Basic Firefly Luciferase Expression Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgl3-basic firefly luciferase expression vector/product/Promega
    Average 90 stars, based on 1 article reviews
    pgl3-basic firefly luciferase expression vector - by Bioz Stars, 2026-03
    90/100 stars

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    The essential region, which regulates LINC00460 transcription in response to irradiation, is located −145 and −44 bp upstream of LINC00460. (A) A schematic representation of the full-length LINC00460 promoter and pGL3-basic, inserted upstream of the luciferase reporter plasmid. The indicated plasmids were transfected into HCT116 cells. Luciferase assays were performed according to the manufacturer’s instructions. The percentage of luciferase activity elicited by a particular deletion construct is presented relative to the activity of the full-length LINC00460 promoter-luciferase construct. Each bar represents the mean and SD of at least three independent experiments. Deletion of −2576/+66-bp caused a moderate enhancement of luciferase activity, whereas deletions of −352/+66-, −240/+66-, −819/+66-, −145/+66-, and −44/+66-bp caused modest-to-severe decreases in luciferase activity. (B) Mutations at the −145/−44-bp region of the LINC00460 promoter. (C) Luciferase expression is depicted as percent intensity, relative to the expression of the −145/−44-bp construct. All data are presented as the means ± SDs (n = 3). *P < 0.05

    Journal: Toxicology Research

    Article Title: Suppression of LINC00460 mediated the sensitization of HCT116 cells to ionizing radiation by inhibiting epithelial–mesenchymal transition

    doi: 10.1093/toxres/tfaa010

    Figure Lengend Snippet: The essential region, which regulates LINC00460 transcription in response to irradiation, is located −145 and −44 bp upstream of LINC00460. (A) A schematic representation of the full-length LINC00460 promoter and pGL3-basic, inserted upstream of the luciferase reporter plasmid. The indicated plasmids were transfected into HCT116 cells. Luciferase assays were performed according to the manufacturer’s instructions. The percentage of luciferase activity elicited by a particular deletion construct is presented relative to the activity of the full-length LINC00460 promoter-luciferase construct. Each bar represents the mean and SD of at least three independent experiments. Deletion of −2576/+66-bp caused a moderate enhancement of luciferase activity, whereas deletions of −352/+66-, −240/+66-, −819/+66-, −145/+66-, and −44/+66-bp caused modest-to-severe decreases in luciferase activity. (B) Mutations at the −145/−44-bp region of the LINC00460 promoter. (C) Luciferase expression is depicted as percent intensity, relative to the expression of the −145/−44-bp construct. All data are presented as the means ± SDs (n = 3). *P < 0.05

    Article Snippet: Various regions of the LINC00460 promoter that were cloned into a pGL3 basic vector (Promega, Madison, WI, USA) expressing the firefly luciferase reporter gene were constructed as described previously [ 25 , 26 ].

    Techniques: Irradiation, Luciferase, Plasmid Preparation, Transfection, Activity Assay, Construct, Expressing